Upasana Roy is in the Department of Biochemistry and Molecular Biophysics, Columbia University Irving Medical Center, New York, New York 10032, USA. Eric C. Greene is in the Department of Biochemistry ...

In the double helix structure of DNA, thymine forms a base pair with adenine through two hydrogen bonds. This specific pairing is known as complementary base pairing and is essential for the stability ...

Antisense oligonucleotides (ASOs) are small, single-stranded molecules that can suppress the expression of targeted genes and thereby achieve therapeutic effects. However, many ASO-based drug ...

We now have billions of identical copies of a stretch of DNA 2,000 to 4,000 base pairs long. From these we need to create copies with special bases that our machines will be able to read. Instead of ...

CRISPR-Cas9 has been editing the human genome for the past decade. Although the types of edits have varied, an unsolved challenge has been the integration of large pieces of DNA without ...