Nature

Degenerate oligonucleotide-primed polymerase chain reaction (DOP-PCR) and amino linking, a series of amplification reactions used to create representations of each clone (1).

First, the BAC clone DNA is denatured into single stranded templates. Each of the three DOP-PCR primers used in the next step is flanked by known 5' and 3' anchor sequences with six random nucleotides ...