This lab includes a theoretical explanation of how buffers work and how they are made, including a derivation of the Henderson-Hasselbalch equation. Students will use what they have learned to ...
Capillary electrophoresis (CE) is a versatile and automated separation technique that exploits the differential migration of charged analytes under an applied electric field, thereby providing rapid ...
What buffers do: Buffers limit pH changes by using a weak acid and conjugate base pair to neutralize added acids or bases through reversible reactions. Why pKa matters: A buffer’s pKa determines the ...